Paris: Top seed Ashleigh Barty retired injured midway through the second set of her French Open second-round match against Poland's Magda Linette on Thursday.
The Australian, who won the title on her last visit to Roland Garros in 2019, left the court for medical treatment after losing the first set 6-1.
She had struggled with a hip problem during her first-round win and called it quits at 2-2 in the second set on Court Philippe Chatrier.
"It's going to be a little bit tough this week. I think over the weekend we had a bit of a flare-up through my left hip," she said after beating Bernarda Pera in three sets on Tuesday.
Barty also retired injured from the Italian Open during her quarter-final against Coco Gauff last month.
It is Barty's earliest exit from a Grand Slam tournament since a second-round loss at Roland Garros in 2018.
The 25-year-old's withdrawal leaves the tournament already without the world's top three-ranked women's players.
World number two Naomi Osaka stunned the sport by pulling out following a media boycott, saying she has suffered "bouts of depression" for three years.
Third-ranked Simona Halep, the 2018 French Open winner, withdrew before the event with injury.
That leaves world number four Aryna Sabalenka as the top remaining player in the draw, despite the Belarusian never having reached a Grand Slam quarter-final.
Unseeded Linette will face Tunisian 25th seed Ons Jabeur in the last 32.
The world's top three will not be the only notable absences from the third round, with injury-plagued former US Open champion Bianca Andreescu having lost at the first hurdle.
The draw has opened up for the likes of reigning champion Iga Swiatek and history-chasing Serena Williams.
The 39-year-old Williams, still looking to equal Margaret Court's all-time record of 24 Slam titles, is the second-highest seeded player left in her half of the draw behind Sabalenka.
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